Journal: eLife

Article Title: DYRK1A interacts with the tuberous sclerosis complex and promotes mTORC1 activity

doi: 10.7554/eLife.88318

Figure Lengend Snippet:

Article Snippet: Samples were imaged with a confocal microscope (Leica Stellaris 5 PL APO 40 X/1.30 oil objective) or Olympus FV3000 (UPLFLN 40 X/1.30 oil objective).

Techniques: Transfection, Construct, shRNA, Transduction, Knock-Out, Recombinant, Plasmid Preparation, Sequencing, Control, cDNA Synthesis, Software

Journal: iScience

Article Title: Salivary ZG16B expression loss follows exocrine gland dysfunction related to oral chronic graft-versus-host disease

doi: 10.1016/j.isci.2021.103592

Figure Lengend Snippet: Single-cell RNA-seq and Immunofluorescence analysis of HV's MSG confirms RNA and protein expression of ZG16B in serous (S-) and seromucous (SM-) acinar cells (A) Left, Experimental workflow of 10x single-cell RNAseq analysis of normal MSG. After dissociation, cells were captured in microfluidic oil droplets, lysed, sequenced, and analyzed. (B) Right, UMAP embedding of 16,289 high-quality cells that were clustered into 14 populations using the Seurat algorithm. (C) UMAP plots showing the expression of ZG16B (top), MUC7 (middle), and KRT19 across clusters. (D) Dot plots illustrate the expression of ZG16B and known epithelial markers. The color of each plot reflects the average expression level from low (yellow) to high (red), and the size of each dot reflects the percentage of positive cells for each gene. (E) STRING network analysis of genes shared between S-Acinar and SM-Acinar clusters shown in (C) The light blue lines represent database evidence; the purple lines represent experimental evidence; the yellow lines represent text mining evidence; and the black lines represent co-expression evidence. The red dotted line adds a new association between ZG16B and MUC7. (F) Representative immunofluorescence staining pattern of co-expression of ZG16B (green) and MUC7 (red) in S- and SM-acinar cells. DAPI (blue) indicates nucleated cells and KRT19 (Cyan) labels ductal cells. Magnification 40x, scale bar = 50μm. See also and and Figure S3 .

Article Snippet: Tissue sections were scanned using a Nikon A1R confocal microscope (fitted with a Plan Fluor 40x/1.30 oil objective) using the NIS Elements imaging software (Nikon Instruments Inc., Melville, NY).

Techniques: RNA Sequencing Assay, Immunofluorescence, Expressing, Staining